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Temperature stayed pretty consistent throughout the project. The heaters helped the water stay between 70-80°C. The minor changes in temperature could have resulted from the use of the two bar bubblers.

DO levels remained constant throughout Phase 1. DO levels were above 5.0 mg/L so the fish weren't introduced to a lot of stress.

pH was under 7, and since the fish are immature, they may have been introduced to some stress.

With the Big Tank, the temperature stayed within a 22-24°C range (72-75°F). However, with having a smaller environment for the two fish, the temperature of the Experiment could've easily be manipulated by many variables, such as the heater, which was set between 70-80°F, but the temperature of the Experiment mostly resided between 66-69°F.

In this phase, we saw that the the high temperatures of the heaters overcome the amount of additional O₂, and caused the DO to lower. When the concentration of fish waste increased, the bacteria that decomposed the waste increased, resulting in more consumption of O₂ (Glenn Research Center). Once the DO levels reached lower than 5.0 mg/L, the fish was put into additional stress (KY Water Watch). This was also physically observed towards the end of the research in which the DO levels were at it’s minimum. (The tilapia were constantly opening and closing their mouths.)

During Phase 2, the pH of the Big Tank remained between 6.2-6.8, while the Experiment actually increased to having a pH level between 7.2-7.8. That would be because of how most of the chemicals we used were neutral salts, which have pH values around 7.

EC showed a decrease in both the Big Tank and the Experiment, and even share similar patterns, even though the Experiment were introduced to the chemicals.

Sources

The temperature stayed consistent throughout this short phase, just like the temperature in the other phases.

The DO levels still remained lower than 5.0 mg/L, so the fish were still showing signs of stress.

With Phase 3, the pH went up and had a similar range compared to the Experiment, and the fish were less stressed.